Topic: proximity labeling

As a whole, the brain interstitial system (ISS) comprises 15-20% of brain tissue providing a supporting microenvironment that nurtures neuronal and glial cells. Many of the components that make up the brain extracellular fluid are not secreted locally at the brain, but rather synthesized in peripheral organs and then imported to the nervous system via the cerebrospinal fluid (CSF) or the bloodstream. Despite the relevance of the ISS during brain development, profiling the different molecules that compose the extracellular space of the brain has proven to be a daunting task due to the complexit

RNA localization is particularly important in morphologically complex cells such as neurons, where precise protein expression is necessary to achieve highly compartmentalized functions. Emerging evidence suggests that endosomal trafficking could be directly involved in RNA localization and translation in neurons. However, the identity of endosomal-regulated mRNAs, and the functional role of endosomes in controlling subcellular protein synthesis, remain to be understood. By coupling proximity labeling and RNA sequencing, we here report the presence of a rich repertoire of mRNAs associated with