Topic: resting membrane potential

Glioblastoma multiforme (GBM) is a highly therapy-resistant primary brain tumor, especially due to the presence of Glioblastoma Stem Cells (GSCs). In fact, GBM treatment imposes a combination of brain surgery, radiotherapy and chemotherapy with Temozolomide (TMZ) but still fails to completely abolish the tumor. Given this, exploring differentiation induction in GSCs emerges as a promising strategy for GBM treatment. Accordingly, we aimed to investigate the mechanisms that regulate stemness maintenance and differentiation in GBM using Whole-Cell Patch Clamp, Western Blot, Immunofluorescence and

The sodium (Na+)-leak channel (NALCN) maintains neuronal resting membrane potential by conducting a sodium leak current. Pathogenic mutations in the NALCN channelosome complex (NALCN-UNC80-UNC79) are linked to rare developmental and epileptic encephalopathies. However, despite the prevalence of cognitive deficits in NALCN-related disorders, the precise role of NALCN in brain development remains elusive. To address this gap, we investigated the contribution of Nalcn to cortical neural circuit formation using mouse models. Employing Nalcn-GFP reporter mice in conjunction with immunohistochemistr