Topic: RFP

A fundamental goal in neuroscience is to connect an animal's behavior to its neural activity. However, imaging neural activity in a behaving animal presents unique challenges because the animal's movements create motion artifacts that, in the worst cases, cannot be distinguished from neural signals of interest. One approach to mitigating motion artifacts is to image two channels simultaneously: one that captures a calcium sensitive fluorophore, such as GCaMP, and another that captures a calcium insensitive fluorophore such as RFP. In principle, because the calcium insensitive channel contains

The brain allows animals to make dynamic behavioral changes in response to changing internal or external demands. Determining precisely how this happens is challenging because existing methods for recording neural activity perturb natural behavior. With the recent advent of tracking microscopy, the larval zebrafish is an ideal vertebrate model for recording brain-wide cellular-resolution neural activity in freely behaving animals during natural behavior. However, current imaging methods such as structured illumination or light field microscopy, compatible with tracking microscopy, rely on high