Topic: RiboTag

Astrocytes, the most abundant glial cell population in the central nervous system, play a crucialrole in regulating neuronal networks in response to sensory input changes. Despite their pivotalrole, the molecular basis underlying astrocytic involvement remains largely unexplored. Ourstudy aims to develop a conceptual framework of astrocytic translatome, elucidating theircontribution following sensory deprivation. To achieve this, we employed Aldh1l1-cre/ERT2 xRPL22tm1.1Psam (Ribotag) mice and induced astrocytic-specific ribosome labelling throughtamoxifen administration. Sensory deprivation wa

Astrocytes, with their intricate morphology, pose challenges for cell-specific RNA isolation using traditional methods, which often result in loss of RNA localised in distal processes. We tackle this issue by adopting the RiboTag method (Sanz et al 2009) in RPL22tm1.1Psam mice. This technique isolates ribosome-associated mRNA (translatome) cell-specifically from crude tissue lysates through ribosomal HA tagging via cell-type specific Cre expression. Despite the widespread use of RiboTag, its detailed adaptation for complex cells like astrocytes, especially in high-throughput scenarios, remains