Topic: RNA localization

RNA localization is a key mechanism to control protein abundance at the subcellular level. Neurons heavily rely on this process for their proper development and function, but how specific mRNAs reach their site of translation remains poorly understood. Here, we investigated the role of the endosomal pathway in controlling RNA localization and translation in neurons. Through rapid isolation of endosomal compartments followed by RNA sequencing, we first found a vast repertoire of mRNAs associated with endosomes in developing neurons, with functions associated with various organelles and molecula

Gene expression is heavily controlled at the level of mRNA translation into protein, allowing for stimulus-dependent and compartment-specific activation of protein synthesis. In neurons, mRNA transport to dendrites and axons and local translation are crucial for development, repair, synaptic function and memory formation.3’ untranslated regions (3’UTRs) are known for regulation of RNA localization, stability and translation. Past research identified many of the molecular mechanisms and players involved, but we still lack a systematic understanding of how the regulatory information is encoded i

RNA localization is particularly important in morphologically complex cells such as neurons, where precise protein expression is necessary to achieve highly compartmentalized functions. Emerging evidence suggests that endosomal trafficking could be directly involved in RNA localization and translation in neurons. However, the identity of endosomal-regulated mRNAs, and the functional role of endosomes in controlling subcellular protein synthesis, remain to be understood. By coupling proximity labeling and RNA sequencing, we here report the presence of a rich repertoire of mRNAs associated with