Topic: RNAscope

Introduction: Transient receptor potential ankyrin 1 (TRPA1) is a non-selective cation channel expressed in the urocortin 1 (UCN1) positive neurons of the centrally projecting Edinger-Westphal nucleus (EWcp) having modulatory impact on stress adaptation. As post-traumatic stress disorder (PTSD) is triggered by disturbed stress adaptation, we hypothesized the regulatory role of EWcp/UCN1/Trpa1 neurons in PTSD.Methods: Male TRPA1 wild-type (WT) and knockout (KO) mice were exposed to two different models of PTSD. In single prolonged stress (SPS) paradigm, time spent immobility was measured during

Nociceptors are a subset of sensory neurons that relay painful stimuli from the peripheral tissues. Multiple nociceptor subtypes exist and can respond to different thermal, mechanical, or chemical noxious stimuli. Recent findings with transcriptomics have identified gene expression patterns that classify these sub-types by different ion channels including NaV1.7, NaV1.8, NaV1.9, TRPV1, TRPA1, P2X3, HCN and a number of others that have been implicated in pain. In this study, we have identified a method to rapidly generate a novel TRPA1+ human induced pluripotent stem cell (hiPSC)-derived sensor

Advancements in spatial imaging technologies (SIT) have broadened transcriptomic discovery capacity. Yet, development of orthogonal technologies for follow-up translational studies have trailed. We have developed MSIA that utilizes non-temporal, optical barcodes by combining the advantages of RNAscope™ double-Z probes and Hiplex v2 signal amplification technology.Fresh-frozen and FFPE brain coronal sections were collected from C57BL/6J mice of 3 age groups: 1 month, 6 months, and 12 months, respectively. MSIA was executed in 3 cycles, with 100 RNA targets in cycles 1&2 and then up to 4 pro

Acute itch has a clear beneficial purpose, directing our attention and physical response to specific areas of the skin to remove potentially harmful substances and parasites through scratching. However, millions suffer from persistent itch, where excessive scratching instead leads to skin injuries and sleep deprivation. Itch-inducing substances are detected by primary afferents in the skin, which relay the information to the dorsal horn of the spinal cord for further processing. Subsequently, ascending pathways transmit this information to the brain making us aware of the itch. Spinal glyciner

Spinal cord injury immediately impairs movement control and other essential physiological functions. Evidence has shown that a large-scale network including spinal and supraspinal structures is recruited after SCI to promote functional recovery. Orexins (hypocretins), known for promoting wakefulness, arousal, and reward-seeking behaviours, also influence muscular tone, locomotion initiation, and skilled performance, exerting a multi-systemic effect on motor control predisposing for movement execution. Despite the potential relevance of orexin transmission in motor recovery after CNS damage, th

Several data suggest that the RFamide peptides - especially the prolactin-releasing peptide (PrRP) – play a role in the regulation of stress responses, the disturbances of which can cause many diseases, including depression. PrRP was identified as an endogenous ligand of the GPR10 receptor, but can also bind to the NPFF2 receptor. Here we would like to reveal the association between depressive-like behaviour and changes in the PrRP regulation. Fifteen minutes forced swimming test (FST) was used to induce depression-like symptoms in male Wistar rats. On the next day during a 6 min test we ident