Topic: Roxadustat

Stroke is one of the major reasons for death and disability. Insufficient blood flow in stroke interferes the brain from proper oxygenation, leading to the stabilization of hypoxia-inducible factors (HIFs). To determine the role of HIFs in stroke, particularly HIF-2α, an in vivo model called tMCAO (transient middle cerebral artery occlusion) and an in vitromodel with human SH-SY5Y cells was used. On both, Hif-2α deficient and wild type mice, tMCAO was conducted for two hours. In addition to RNA and protein samples, tissue for immunofluorescence microscopy was collected of ischemic and control

Huntington’s disease (HD) and X linked dystonia parkinsonism are basal ganglia diseases, which have in common a decrease in TAF1 expression. Transgenic HD mouse model (R6/1) show diminished TAF1 levels together with an imbalance in usage of an alternative 3’ splicing site of TAF 1, which leads to an increase of isoforms with the long version of exon 5 (TAF1-e5L), which carry a putative prolyl hydroxylase (PHD) dependent degradation motif. This alteration is corrected by SREK1 overexpression, and leads to behavioral and neuropathological recovery of R6/1 mice. We aim to analyze TAF1 splicing va