Topic: sciatic nerve

Vincristine (VCR), an anticancer drug, frequently causes chemotherapy-induced peripheral neuropathy (CIPN). We have reported that macrophages (Mφ)-derived HMGB1 is involved in CIPN induced by paclitaxel in mice, whereas the mechanisms for VCR-induced CIPN (VIPN) remain unclear. We thus examined whether VIPN involves HMGB1 derived from Mφ or Schwann cells in mice. VCR at 0.2 mg/kg was administrated i.p. to mice daily for 10 days with a 2-day break, and nociceptive threshold at the hindpaw was evaluated by von Frey test. An anti-HMGB1-neutralizing antibody (HMGB1-Ab), soluble thrombomodulin (TMα

Aims: To investigate the effects of methylcobalamin (MeCbl) on angiogenesis after peripheral nerve injury.Methods: For in vivo experiments, we used eighteen 6-week-old male Wistar rats. The left sciatic nerve was excised and bridged using the decellularized nerve. Then, rats were randomly divided into MeCbl treated and saline treated groups. At postoperative 7 days, nerves were harvested and evaluated for immunohistochemistry. For in vitro experiments, scratch assay, and tube formation assay were evaluated in the presence or absence of MeCbl using human umbilical vein endothelial cells. Additi

Central nervous system injuries impede motor commands transmission to target muscles. Functional Electrical Stimulation (FES) bypass central motor control by directly stimulating the nerves of paralyzed muscles. Intraneural electrode for FES application have the potential to increase the selectivity and decrease the current threshold for activation compared to extraneural or surface electrodes. This study aims to identify electrical stimulus parameters that optimize the use of intraneural electrodes for neuromuscular FES.Electrical pulses were delivered using a non-commercial multi-channel sti

Neuromodulation techniques offer drug-free management of chronic pain. However, electrical stimulation does not discriminate between sensory and motor fibres, nor between their functional subtypes, and can therefore result in off-target effects (e.g., motor activation). One way to alter nociceptive fibre activity alone is through the selective expression of light sensitive opsins within nociceptive fibres. Thus, light may be used to alter nociceptive activity whilst leaving neighbouring fibres unaffected. We selectively expressed either excitatory (channelrhodopsin; ChR2) or inhibitory (halorh