Topic: SDS-digested freeze-fracture replica labelling

N-methyl-D-aspartate receptors (NMDARs) are key players in synaptic transmission and plasticity underlying learning and memory. Consequently, synaptic dysfunction of NMDARs has been implicated in the pathophysiology of Alzheimer´s disease (AD).Aims: to unravel possible alterations of GluN1, the obligatory subunit of NMDARs, in different excitatory synapses in the hippocampus.Methods: We used histoblot and SDS-digested freeze-fracture replica labelling (SDS-FRL) techniques.Results: Histoblots showed that GluN1 expression was significantly reduced in the caudate putamen, cortex and in the hippoc

Voltage-gated CaV2.1 Ca2+ channels play a crucial role in regulating neurotransmitter release, thus contributing to processes such as learning and memory. Despite their recognised importance in neural function, there is limited information on their potential involvement in neurodegenerative conditions such as Alzheimer's disease (AD). Aims: to explore the impact of amyloid-β (Aβ) pathology on the nanoscale compartmentalization of CaV2.1-GABAB in the hippocampus.Methods: We used histoblot and SDS-digested freeze-fracture replica labelling (SDS-FRL) techniques in the CA1 field of the hippocampus