Topic: secretagogin

Secretagogin, a Ca2+-sensor protein, can trigger signal transduction cascades promoting vesicular exocytosis in an activity- and Ca2+-dependent manner. Even though a role for secretagogin in vesicle trafficking was hypothesized, it’s structural motifs that govern any such function remain unknown. In silico search of the secretagogin interactome was used to select consensus protein hits from open-label databases. X-ray crystallography was employed to map the interaction motif. Multiple immunostaining coupled to superresolution microscopy aided the localization of recombinant structural modules

Higher order sensory information is typically relayed through the posterior thalamustowards the cortex. Using a transgenic mouse strain, we identified a neuronpopulation in the posterior thalamic nuclear group, based on their transient embryonicexpression of secretagogin, a calcium sensor protein. Anterograde tracing wasperformed by using adeno-associated viral vectors carrying the mCherry transgene in12-16 weeks old Scgn-Cre mice and imaging was performed after standardimmunohistochemistry on a ZEISS 780 CLSM platform. Other animals received AAVparticles carrying Cre-dependent DREADD expressi