Topic: seizure-like activity

Epilepsy is a chronic neurological disorder characterized by recurrent seizures resulting from abnormal electrical activity in the brain. All experimental models for seizure generation are based on the administration of drugs (e.g. pilocarpine), on artificial increase in the concentrations of extracellular K+ (Ko) or on direct electric stimulation, to name a few. However, all these approaches may not accurately reflect the in vivo conditions, where otherwise normal neuronal activity can generate occasional seizures. It is thus important to better understand the pathological changes that may be

Neuronal networks derived from human induced pluripotent stem cells (hiPSCs) have gained attention for their potential use in in vitro modeling of epilepsy and seizure-like electrophysiological activity. Similar to in vivo animal models, seizure-like activity can be induced in these neuronal cultures using kainic acid (KA), direct agonist of the glutamate receptors. However, to establish more relevant in vitro model using KA, better understanding of KA mediated acute and prolonged effects on human neuronal networks is required. In this study, we investigated both acute and long-term KA concent

Seizure-like activity (SLA) results from synchronous neuronal activity in the mouse hippocampal subregions, namely: CA1, CA3 and DG. While many studies have proved the anticonvulsant properties of the administration of anti-epileptic drugs, such as diazepam (DZP), a GABA-A receptor agonist, and carbamazepine (CBZ), a sodium channel blocker, their role in modulating the oscillatory patterns within these three regions remains unclear. Furthermore, the role of oscillatory dynamics in the pre-ictal period in predicting the emergence of a seizure events require further investigation. In our study,