Topic: serotonergic projections

Despite the well-demonstrated modulatory effect of serotonin on spinal motor circuits, the specific roles and identity of the involved serotonergic neurons remain unclear. This ambiguity owes to the anatomical and neurochemical heterogeneity of the medullary brainstem regions housing the spinally-projecting serotonergic neurons, and to the limited number of studies investigating their connectivity and function cell-specifically.Here, we selectively target the midline serotonergic neurons of the caudal medulla (mCM-5HT) in the mouse to explore their output connectivity in the spinal cord and th

The dorsal raphe nucleus (DRN) modulates diverse behaviors and brain functions through its extensive serotonergic projections across the forebrain. Recent studies showed that vertebrate DRN is composed of distinct types of molecularly defined and topographically organized populations of neurons that are evolutionarily conserved. However, how and what do these topographically organized DRN neuron ensembles communicate to the forebrain, and the role of DRN in forebrain computations and animal behavior remain elusive. Here, we employed two-photon calcium imaging in juvenile zebrafish to investiga