Topic: Sholl analysis

Aims: Metabolic profile impacts on tissue development including neurons in brain, yet the full repertoire of metabolic signals that shape developing neuronal architecture remain mysterious. Our aim is to clarify the metabolic crosstalk that coordinates the early acquisition of the neurite complexity in developing neurons. Methods: To investigate the factors involved in metabolic switch, we used transcriptomics analysis on the sensory neurons cultured in different metabolic demand. IPA analysis was used to interpret the transcriptomics data. We applied MEME-ChIP method on comprehensive motif an

Human brain organoids are 3D self-assembling constructs obtained from human pluripotent stem cells, differentiating, and recapitulating the structural and functional complexity of the human brain. Quantitatively describing the cellular spatial arrangement within these constructs and their morphology is important to assess their structural resemblance to the human brain, and thus effectively demonstrating their ability to recapitulate the brain salient microstructural features. Thanks to pluripotent stem cell technology, brain organoids obtained from cells of healthy and diseased patients will

PhospholipaseC γ2 (PLCG2) is known to have direct link with genetic risk factors for Alzheimer’s like dementia (AD). Previous work showed its action in AD is regulated through microglia. However, the synaptic/ electrophysiological correlates are elusive. In present study, WT mouse were transfected with sh-RNA of LV-PLCG2 (U6 promotor, GFP as reporter; shRNA) or scrambled (used as control; shNT). Whole-cell voltage-clamp recordings were performed on acute slices with glass pipette (<3-4MΩ) at -70mv, followed by morphometry. Two-tailed Mann-Whitney test was used for statistics unless otherwis

Introduction: Rett syndrome (RTT) is a rare neurodevelopmental disorder caused in 95% of cases by mutations in the X-linked methyl CpG-binding protein 2 (MeCP2) gene. Although apparently normal at birth, RTT patients manifest a regression phase between 6-18 months of age characterized by loss of speech, impaired motor functions, seizures and intellectual disabilities. Clinical and animal studies suggested that metabolic pathways might be affected and contribute to RTT pathophysiology. Autophagy is a catabolic process involved in the degradation of cellar debris and recently found to regulate a

Intellectual disability and autism spectrum disorders resulting from MECP2 gene alterations, such as Rett syndrome (RTT) and MECP2-duplication syndrome (MDS), present challenges in understanding the complex links between genetic alterations and cognitive deficits. Previous animal-based studies, though insightful, face translational barriers due to evolutionary differences in brain development between species, hindering direct application to humans.To address this, we established an innovative in vivo human neuronal model for RTT and MDS, utilizing patient-derived induced pluripotent stem cells