Topic: single-cell resolution

Savina Cammalleri discusses extending DISCO, a digital microfluidic platform for single-cell omics, to protein analysis. The work uses controlled handling of individual cells and samples to make proteomic measurements more efficient and scalable. The presentation considers how a flexible microfluidic approach can support studies of variation between cells and the molecular processes underlying that variation. Its goal is to broaden the experimental tools available for biological measurements at single-cell resolution. Cammalleri is a doctoral researcher in biomedical engineering at the Univer

Understanding the fundamental gene regulatory mechanisms underlying addiction and related behaviors could facilitate more effective treatments. We discuss our work using multi-omics methods to provide mechanistic and functional insights into how addiction perturbs gene regulatory programs in the rat brain, with single-cell resolution.

Ultra-low-cost, easily implemented and flexible two-photon scanning microscopy modification offering a several-fold expanded three-dimensional field of view that also maintains single-cell resolution. Application of our system for imaging neuronal activity has been demonstrated on mice, zebrafish and fruit flies. Website: https://github.com/BadenLab/nTCscope

A fundamental problem in neuroscience is how neuronal activity in the brain generates organized and stable behaviors across multiple timescales. Recent research in many model organisms, e.g. in monkeys, led to the observations that the neuronal encoding of individual actions, such as preparation for and execution of arm movements, is achieved by large neuronal populations but often lies on low-dimensional manifolds. However, it is largely unclear if such manifolds also coordinate higher dimensional activity patterns that correspond to simultaneously executed fine-tuned movements. In order to a