Topic: single-nuclei RNA sequencing

Erythropoietin (EPO), a potent growth factor, named after its role in erythropoiesis, has been shown to not only be produced in the brain, but also to have significant effects in improving cognitive performance. The mechanisms by which EPO acts are yet to be fully explored, including the transcriptome changes elicited by it in the oligodendrocyte lineage. Here we explore these changes after a 3-week EPO/Placebo treatment using single-nuclei RNA sequencing. A total of 12.043 nuclei were classified into 5 cell types (oligodendrocyte precursor cell – OPC, committed oligodendrocyte precursor – COP

Alzheimer’s disease (AD) is a complex disorder involving multiple genetic and non-genetic factors leading to cognitive decline and the accumulation of protein aggregates. Recently, we found that the epi-genetic regulation of Peptidase M20 Domain Containing 1 (PM20D1) modulates AD risk in humans and disease progression in mice through a still unknown mechanism. PM20D1 is an enzyme that facilitates the conjugation of N-acyl amino acids (NAAAs). However, whether NAAAs exert similar protective effects in AD remains unknown. To investigate this hypothesis, we NAAA-treated a series of AD models, and

The entorhinal cortex (EC) plays an important role in memory and navigational processes. The EC is part of the three-layered allocortex and interestingly, the structure in several cortical areas of the reptilian ancestor of mammals was three-layered as well. The EC is shown to be affected in Alzheimer’s disease already at preclinical stages. Furthermore, spatial disorientation is a characteristic cognitive symptom in patients suffering from Alzheimer’s disease. To learn about evolutionary similarities of the EC, we carried out single-nuclei RNA sequencing analysis on salmon telencephalon, frui