Topic: single-nucleus RNA-sequencing

A spinal cord injury (SCI) triggers a multifaceted series of secondary injuries, involving inflammation, cell death, demyelination, and scar formation. How the individual types and subtypes of cells of the central nervous system coordinate to mediate this response remains unclear. While much of our current knowledge is derived from rodents, therapies effective in these models have not translated successfully to clinical trials. Understanding molecular responses across species—lower vertebrates, non-primate mammals, and primates—is crucial for formulating effective human treatments. We aim to u

The amygdala, or an amygdala-like structure, is found in the brains of all vertebrates and plays a critical role in survivaland reproduction. However, the cellular architecture of the amygdala and how it has evolved remain elusive. Here, we generated single-nucleus RNA-sequencing data for more than 200,000 cells in the amygdala of humans, macaques,mice, and chickens. Abundant neuronal cell types from different amygdala subnuclei were identified in all datasets. Cross-species analysis revealed that inhibitory neurons and inhibitory neuron-enriched subnuclei of the amygdala were well-conserved i