Topic: snRNA-seq

How do neurons in the medial entorhinal cortex (MEC) organize themselves during development to establish networks involved in complex functions like learning and memory? From developmental studies of cortical and subcortical structures, one transcription factor called Bcl11b (encoding Ctip2) is responsible for neuronal differentiation and axonal pathfinding. Interestingly, in the MEC, Bcl11b is expressed by Reelin-positive (Reln+) projection neurons in layers 2 and by intracortical projection neurons in layer 5a . Because Bcl11b is widely expressed by these early born MEC neurons, we hypothesi

Autism spectrum disorders (ASD) are associated with a high genetic heterogeneity, yet for many of the cases, the underlying molecular causes remain elusive. While several studies have elucidated some distinct patterns, fundamental questions persist: How do mutations dispersed across numerous genes lead to the development of a phenotypically similar disorder? Essentially, it remains largely unclear whether mutations in distinct genes lead to similar molecular and cellular states within the brain. Thus, it is important to identify molecular mechanisms responsible aiming to decipher points of con

Painful diabetic neuropathy (pDN) is a serious, chronic complication affecting about 10-20% of diabetes mellitus (DM) patients, but the underlying cause remains unknown. Hints from risk factor assessment and literature point towards a role of oligodendrocyte and myelin homeostasis. Along this line, the main aim of the project is to identify adaptations occurring in the oligodendrocyte lineage due to the metabolic imbalances triggered by DM and determine which of these adaptations are crucial for the development of neuropathic pain. The high-fat high-sugar diet-induced type 2 DM mouse model is

Tuberous sclerosis complex (TSC) is a genetic disorder where many patients affected by the disease suffer from refractive epilepsy. To alleviate related symptoms, the patients must undergo surgery to resect the epileptogenic lesion. Obtaining this tissue and instantly flash freeze it provides a unique opportunity to gain insight to the pathogenesis of TSC.Thus, we embarked on an in-depth unbiased characterization of the transcriptomic landscape, cellular identify, and potential epileptogenic networks of frontal cortical tubers of pediatric TSC using snRNA-seq on resected epileptic tissue and c