Topic: Tandem Mass Tag

Mechanistic target of rapamycin (mTOR) is a serine/threonine kinase that regulates fundamental cellular processes including growth control, autophagy and metabolism. mTOR constitutes the catalytic subunit of two signalling complexes: mTOR complex 1 (mTORC1) and 2 (mTORC2). Manipulating mTORC1 signalling in animal models has shown that this pathway has key roles in neurogenesis, and mTORC1 hyperactivation during neurodevelopment causes a group of disorders known as mTORopathies. mTORopathies typically involve brain malformations and neurological manifestations like epilepsy. One example is tube

Amblyopia arises from neuronal adaptation due to abnormal experience of one eye during a critical period of visual development. The molecular mechanisms underlying amblyopia are not characterized. To investigate this process, we used the monocular deprivation experimental paradigm in young rats to induce plasticity of neuronal excitability in dorsal lateral geniculate nucleus (dLGN) neurons (Duménieu et al., BioRxiv 2023). Employing a proteomic approach, we identified activity-dependent dynamic change in the components of dLGN neurons. By microdissection we isolated deprived and spared regions