Topic: tiagabine

Intractable chronic itch is resistant to antihistamines, and difficult to treat, which significantly reduces quality of life. Here, we focused on GABA transporter 1 (GAT1) whose blockade might have an antipruritic effect by increasing endogenous GABA.The allergic contact dermatitis model of chronic itch was generated by applying 1-fluoro-2,4-dinitrobenzene (DNFB) onto the shaved neck skin using ICR male mice. Five days after sensitization by topical application of 50 µL 0.5% DNFB solution, mice were challenged repeatedly with 30 µL 0.25% DNFB solution on day 1, 3, 5, and 7. Scratching frequenc

The human γ-aminobutyric acid (GABA) transporter 1 (hGAT-1) is responsible for clearing GABA from synapse into neurons and astrocytes.Its mutations are known to impair protein folding and trigger epilepsy.Such folding defects are amenable to rescue by chemical and pharmacological chaperones.Our aim is to elucidate molecular features of two novel disease variants, in HEK293 cells (in vitro) and Drosophila melanogaster (in vivo).Deglycosylation experiments (endoglycosidase H(Endo H)) revealed distinct expression patterns for wild type hGAT-1 and the mutants: ER-resident proteins are core-glycosy

Gamma-aminobutyric acid (GABA) plays a key role in learning and brain plasticity by regulating functional connectivity and oscillatory activity in local circuits and global brain networks. Altered cortical inhibition is associated with perceptual and learning impairments in psychiatric disorders such as schizophrenia. Despite the importance of GABAergic inhibition for brain function, we lack a mechanistic understanding of the inhibitory network dynamics that support adaptive behaviour from training. To unravel these mechanisms, we developed a progressive 3-stimuli touchscreen orientation discr