Topic: transendothelial electrical resistance

The vasculature of the brain is made up of specialized endothelial cells that form a tight blood-brain barrier (BBB). Weakening of the junctional proteins can lead to BBB disruption. This can have detrimental effects on patients’ health, as the homeostatic environment of the brain becomes compromised.The MIMETAS OrganoPlate® is a microfluidic platform that enables perfused, 3D culture of up to 96 tissues in parallel in a membrane-free manner. The microfluidic channels allow growth of perfused tubular endothelial structures. Furthermore, the system allows for automation, enabling higher through

SYNGAP1-related encephalopathy is a rare neurodevelopmental disease characterized by diverse genetic mutations and phenotypic features. It is not known whether the blood-brain barrier (BBB) is affected in SYNGAP1 patients, therefore, this study aimed to establish a human induced pluripotent stem cell (hiPSC)-based BBB model of the SYNGAP1 disorder and identify relevant functional and molecular signatures. To establish the BBB models, SYNGAP1 knockout (KO), patient-derived hiPSC lines (Leu323Arg) and isogenic controls were differentiated into brain capillary endothelial-like cells and seeded on

Acute pancreatitis (AP) is an inflammatory gastroenterological disease, during which about 4% of all patients develop disturbance in consciousness. Besides this sypmtom among the severe AP cases 10 % show serious neurological involvement manifesting in pancreatic encephalopathy. Earlier research from our laboratory showed blood-brain barrier (BBB) permeability elevation in a rat non-invasive AP model. Now our goal was to identify potential BBB injury in pancreatitis patients. For this study we used serum from mild, moderate and severe AP patients to identify BBB opening by measuring neuron spe

Disruption of the blood-brain barrier (BBB) is a key factor in the development of stroke. N,N-dimethyltryptamine (DMT) is a hallucinogen alkaloid widely found in plants and animals. Beneficial effects of DMT was described in a rat stroke model, but BBB functions were not studied. Our aim was to examine the effect of DMT on the functions of the BBB following oxygen-glucose deprivation and reoxygenation (OGDR) in a rat primary BBB co-culture system.To model stroke in vitro, OGD was introduced for 6 hours on the BBB model, followed by reoxygenation for 24 hours with or without the addition of DMT

BBB disruption is a disease-driving feature of several neurological disorders, including ALS, yet today no treatment exists to repair the BBB. Here, we screened the effect of a subcommissural organ-spondin-derived peptide (NX210c), known to promote functional recovery in several models of neurological disorders, on BBB integrity.Mouse endothelial cell (EC) monolayers and human BBBs (EC, astrocytes, pericytes) in static or microfluidic conditions, were treated with NX210c (1-100μM) or its vehicle. In mouse EC, NX210c induced a transient increase in occludin levels after 24h treatment (+37%, 100