Topic: ultracentrifugation

The dynamics of biological systems depend on complex cell-cell interactions. Extracellular vesicles (EVs) are nanosized vehicles of intercellular communication which shuttle several molecules from cell to cell, thus influencing relevant biological processes. Previous literature based on EV isolation from cell culture medium highlighted how EVs in the peripheral nervous system could be important mediators in the molecular dialogue between neurons and Schwann cells. Here, we aim to extract EVs directly from peripheral nerve tissue, as this would give the unique opportunity to characterize EVs fr

At present, no effective therapies are available for traumatic spinal cord injury (SCI). Following the initial trauma, persistent inflammation exacerbates neuronal and functional losses. Our recent findings highlight the potential of acute treatment with MSC-derived extracellular vesicles (MSC-EVs) in mitigating inflammation and its consequential damages after SCI. However, with numerous protocols available for EV preparation from MSC-derived secretomes, we sought to identify the most efficacious method for generating EV preparations capable of reducing the pro-inflammatory polarization of mic