Dense synapse-level reconstruction of brain tissue with super-resolution light microscopy
Julia Lyudchik, Tavakoli Mojtaba, Januszewski Michal, Agudelo Nathalie, Vorlaufer Jakob, Vistunou Vitali, Oliveira Barbara, Cenameri Alban, Sommer Christoph, Novarino Gaia, Jain Viren, Danzl Johann · FENS Forum 2024
Mapping the complex and dense arrangement of cells and their connectivity in brain tissue requires volumetric imaging at nanoscale spatial resolution. Light microscopy excels at visualizing specific molecules and individual cells, but dense, synapse-level circuit reconstruction has not yet been achieved.We have developed a light microscopy-based approach to visualize and analyze connectomes in mammalian brain. We base our technology, light-microscopy based connectomics (LICONN) on expansion microscopy to visualize the ultrastructure of nervous tissue, and show traceability of even the finest o
ePoster · Neuroscience