Topic: V1 interneurons

To parse the neural basis of motor behavior, we leveraged the unique swim-to-walk transition of the Xenopus metamorphosis. This transition enabled us to dissect the spinal circuitry underlying tail and limb-based movement. We combined a robust behavioral assay with molecular cell-type profiling in wild-type, and then loss-of-function mutant, tadpoles to evaluate how neuronal diversity scaled with and contributed to the observed increase in movement complexity.We devised a high-speed behavioral setup to track frog’s body parts, quantify complex movement, and create locomotor profiles correlated

Neural circuits in the spinal cord are composed of diverse sets of interneurons that play a crucial role in shaping motor output. Despite progress in revealing the cellular architecture of the spinal cord, the extent of cell type heterogeneity within cardinal interneuron classes remains unclear, hindering our understanding of the organization of the spinal motor infrastructure. Through a focus on a single cardinal class of spinal interneurons, here we present a comprehensive atlas of V1 interneuron diversity across postnatal development using single-nucleus transcriptomics. We find that cell-t