Topic: VAChT

ePoster: 2 ePosters

Nanoscopic distribution of VAMP2 and VAMP7 in striatal cholinergic varicosities and their respective localization with VAChT and VGLUT3 in synaptic vesicles

Mazarine Desplanque, Paola Cristofari, Odile Poirel, Alison Hébert, Sylvie Dumas, Véronique Fabre, Stéphanie Daumas, Nicolas Pietrancosta, Salah El Mestikawy, Véronique Bernard · FENS Forum 2024

AimStriatal cholinergic interneurons (CINs) use both acetylcholine (ACh) and glutamate (Glu) to regulate the striatal network. CINs express vesicular transporters for acetylcholine (VAChT) and Glu (VGLUT3). We recently demonstrated, using super resolution STimulated Emission Depletion microscopy (STED) microscopy, that part of cholinergic synaptic vesicles (SVs) contains both VAChT and VGLUT3, whereas others express only VAChT or only VGLUT3. CINs SVs are thus able to release either ACh or Glu or both. However, the link between neurotransmitters accumulation into SVs and their final exocytosis

ePoster · Neuroscience

Plastic changes of neurons and pericellular synapses in spinal cord after peripheral nerve injury

Katsuyuki Konishi, Toru Iwahashi, Toshiki Shimada, Yoshiaki Yoshimura, Atsushi Kamata, Mai Konishi, Arisa Kazui, Ryoya Shiode, Satoshi Miyamura, Kunihiro Oka, Seiji Okada, Hiroyuki Tanaka · FENS Forum 2024

Aims: Plastic changes of neurons in spinal cord after peripheral nerve injury remain to be elucidated. The aim of this research is to clarify plastic changes of neurons and pericellular synapses in lumbar spinal cord after sciatic nerve transection.Methods: Neurons and pericellular synapses in lumbar spinal cord of rats were analyzed 6 weeks after sciatic nerve transection. The axonal volume of cortical neurons was labeled with biotinylated dextran amine, the volume of motoneuron soma with Fluoro-Gold, cholinergic interneurons with vAchT, and pericellular synapses with vGlut1 and vAchT. They w

ePoster · Neuroscience