Topic: VAMP7

Glioblastoma is an aggressive primary brain cancer with poor prognosis and limited therapeutic options. In tumors, glioblastoma cells control their microenvironment through the secretion of proteins and other factors. Work from our lab has identified a new unconventional secretion mechanism that is dependent on VAMP7, a tetanus neurotoxin insensitive v-SNARE. We have shown that Endoplasmic Reticulum (ER) and mitochondrial fragments can be incorporated into late endosomes and can later be released by an unconventional secretory mechanism. This process is more efficient in the absence of degrada

AimStriatal cholinergic interneurons (CINs) use both acetylcholine (ACh) and glutamate (Glu) to regulate the striatal network. CINs express vesicular transporters for acetylcholine (VAChT) and Glu (VGLUT3). We recently demonstrated, using super resolution STimulated Emission Depletion microscopy (STED) microscopy, that part of cholinergic synaptic vesicles (SVs) contains both VAChT and VGLUT3, whereas others express only VAChT or only VGLUT3. CINs SVs are thus able to release either ACh or Glu or both. However, the link between neurotransmitters accumulation into SVs and their final exocytosis

Intracellular membrane protein trafficking is crucial for both normal cellular physiology and cell-cell communication. It is now established that intracellular trafficking not only uses the conventional secretory pathway but also an alternative secretion pathway able to bypass the need for passage through the Golgi apparatus, which is collectively termed unconventional protein secretion. One of the major actors of this pathway is the vesicular SNARE VAMP7, which is involved in the secretion of extracellular vesicles of late endosomal origin called exosomes. It was recently shown that exosomes