Topic: VGLUT3

AimStriatal cholinergic interneurons (CINs) use both acetylcholine (ACh) and glutamate (Glu) to regulate the striatal network. CINs express vesicular transporters for acetylcholine (VAChT) and Glu (VGLUT3). We recently demonstrated, using super resolution STimulated Emission Depletion microscopy (STED) microscopy, that part of cholinergic synaptic vesicles (SVs) contains both VAChT and VGLUT3, whereas others express only VAChT or only VGLUT3. CINs SVs are thus able to release either ACh or Glu or both. However, the link between neurotransmitters accumulation into SVs and their final exocytosis

Environmental stress plays a key role in the development of psychiatric disorders, and also has a negative impact on sleep integrity. Insomnia and sleep disturbances are core symptoms observed in patients with stress-related disorders. However, stress reactivity is the result of a combination of genetic and environmental influences that ultimately impacts one’s physiology and behavior in a variety of ways. In this project we will focus on stress susceptibility and how it could trigger psychiatric diseases in a peculiar gene-environment interaction.Studying glutamatergic neurotransmission, we d

One of the main targets of the vesicular glutamate transporter type 3 (vGluT3)-expressing median raphe neurons, is the dorsal hippocampus, a key node of the brain’s episodic memory circuit. We uncovered previously that the raphe-hippocampal glutamatergic connection selectively and highly efficiently activates certain types of hippocampal inhibitory neurons at very short latency. However, the function of this powerful inhibition-targeting modulation is still elusive. In this study, we combined the selective tagging of either vGluT3 MR neurons or their hippocampus-projecting subgroup and high-de