Topic: whole-cell patch-clamp recording

Hypoinsulinemia is a pathological consequence of diabetes mellitus that can stimulate the development of neurodegenerative processes in the brain due to dysfunction of insulin signaling cascades into neurons and impaired synaptic plasticity properties. We used the whole-cell patch-clamp recording of evoked glutamatergic excitatory postsynaptic currents (eEPSCs) with the local stimulation of presynaptic axon to investigate the insulin (100 nM) effect on the paired-pulse plasticity at cultured hippocampal neurons under hypoinsulinemia (4 days insulin-free media incubation).At control normoinsuli

Parenting is essential for the well-being of newborns. Laboratory virgin female mice exhibit parental behaviors, including pup retrieval, a phenotype that becomes progressively more efficient when exposed to multiple pups. Yet, the neuronal substrate that supports such behavioral improvement remains unexplored. Previous studies highlight the lateral habenula (LHb) as a substrate for parental behaviors in virgin females. Notably, a population of LHb cells innervated by the excitatory bed nucleus of the stria terminalis (BNST) shows increased activity during pup retrieval. Consistently, perturba