<SUB>​</SUB>DIFFERENTIATION OF HUMAN OLFACTORY MUCOSAL ECTOMESYNCHYMAL STEM CELLS INTO MOTOR NEURON SUBTYPES AFTER EX VIVO EXPANSION

The Human Olfactory ecto-mesenchymsl stem cells (hOEMSCS) are regareded as a suitable source for neural revitalization studies due to comparitive accessibility. The current study aimed to induce differentiation of the hOEMSCS into functional motor neuron like cells MNLCS) using the neurosphere technique and promote neurodegeneration by stimulating local neurogenesis in the adult spinal cord slice culture.
The isolated cells were cultured in serum-free medium supplementedwith 2% B27 to stimulate the formation of floating neurospheres. To motor neuron induction, the neurospheres were induced in two steps first by Shh and RA and, then with GDNF and BDNF administration. Tbe functional properties of MNLCs were evaluated by examining synaptic vesicle release and calcium signaling using a co-culture system , FM1-43 dye staining, a d the calcium indicator FLUO-2. Statistical analysis was performed using SPSS software.
The olfactory-derived stem cells exhibited high expression levels of neural progenitor markers following neuroshere culture. Im.unocytochemistery findings confirmed the ChAT positive cells was ​observed at the end of the induction stage in the treated vroup. The functionality of MNLCs was xemonstrated by FM1-43, intracellular calcium ion shift
and co-culture with C2Cl2. We co-cultivaged hOEMSCS with adult rat organotype spinal cord slice culture in vitro.
The fingings proved the hOEMSCs, following proper isolation, can successfully diffefentiate into functional motor neurons under nereospbere culture conditions and in the presence of appropriate neurotrophic factors. Tbese cells may therefore relresent a promising candidate for neuronal tissue engineering and cell-based therapeutic approache.