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APPLICATION OF PHYSIOLOGICAL COMPOUNDS TO DIFFERENTIATE WHARTON'S JELLY-DERIVED HUMAN MESENCHYMAL STEM/STROMAL CELLS INTO NEURAL LINEAGE
Weronika Maksymiukand 4 co-authors
Mossakowski Medical Research Institute Polish Academy of Sciences
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS04-08PM-182
Presentation
Date TBA
Board: PS04-08PM-182
Event Information
Poster Board
PS04-08PM-182
Poster
View posterAbstract
Mesenchymal stem/ stromal cells (MSCs) are used in various medical treatments due to their immunomodulatory and regenerative properties. Stem cell therapy is one of the most promising approaches for neurodegenerative diseases, as MSCs are known to have protective effects in brain disorders caused by pathological conditions or injuries. As in the central nervous system (CNS), glial cells naturally support neuronal function, the study aimes to develop an effective protocol for differentiating MSCs derived from Wharton's jelly of human umbilical cord (WJ-MSCs) into glial cells. WJ-MSCs were isolated within 24 hours after caesarean delivery. After migrating out of the bioptates, the cells were cultured in serum-free media supplemented with factors involved in neurodevelopmental processes (PDGF-AA and T3). Neural commitment was verified by means of morphological examination, molecular biology and immunocytochemical methods. The cells subjected to conditions that promote gliogenesis exhibited morphological changes and upregulation of NG2 gene expression, as well as were immunolabelled with a panel of stem and neural markers (PDGFRalpha, NANOG, GFAP). The preliminary results suggest that WJ-MSCs have the capacity to undergo neural differentiation, paving the way for the development of efficient protocols to derive cells that could potentially repair damaged nervous tissue.
Acknowledgements: Supported by National Science Center (NCN) in Poland, grant: 2022/47/O/NZ4/01161
Acknowledgements: Supported by National Science Center (NCN) in Poland, grant: 2022/47/O/NZ4/01161
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