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ePoster
INTEGRATING MICROGLIA INTO A PRIMARY CORTICAL TRI-CULTURE TO STUDY NEURO-INFLAMMATION
Almar Kuipersand 3 co-authors
Danone Global Research & Innovation Center
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS04-08PM-155
Presentation
Date TBA
Board: PS04-08PM-155
Event Information
Poster Board
PS04-08PM-155
Poster
View posterAbstract
Neuroinflammation plays a major role in brain disorders like Alzheimer’s disease, stroke, and traumatic brain injury. Microglia are essential immune mediators in these conditions, but traditional primary cortical tri-cultures often lack microglia, limiting their relevance. To address this, we integrated isolated microglia into an established tri-culture of neurons, astrocytes, and myelinating oligodendrocytes, forming a quadruple in vitro model.
Microglia were isolated from mixed glial cultures and added to 7-day-old tri-cultures. Upon isolation, microglia were kept in presence of supplements (M-CSF, TGF-β and cholesterol) for microglial support in serum-free environments. The quadruple culture was monitored for 14 days using live-cell imaging and immunocytochemistry. To evaluate immune response cultures were exposed to LPS. Microglial numbers peaked at day 7, coinciding with less cell death. While integration of microglia did not impact neuronal or astrocytic development, it may have stimulated the differentiation of oligodendrocytes.
In summary, we developed a quadruple culture model incorporating microglia that serves as a promising tool for screening nutritional compounds aimed at reducing neuroinflammation.
Microglia were isolated from mixed glial cultures and added to 7-day-old tri-cultures. Upon isolation, microglia were kept in presence of supplements (M-CSF, TGF-β and cholesterol) for microglial support in serum-free environments. The quadruple culture was monitored for 14 days using live-cell imaging and immunocytochemistry. To evaluate immune response cultures were exposed to LPS. Microglial numbers peaked at day 7, coinciding with less cell death. While integration of microglia did not impact neuronal or astrocytic development, it may have stimulated the differentiation of oligodendrocytes.
In summary, we developed a quadruple culture model incorporating microglia that serves as a promising tool for screening nutritional compounds aimed at reducing neuroinflammation.
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