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ELECTROPHYSIOLOGICAL ANALYSIS ON ORGANOTYPIC BRAIN SLICE CULTURES UPON <EM>AP2M1</EM> MODULATION
Sebastián Maruriand 7 co-authors
Uniklinik RWTH Aachen
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS05-09AM-421
Presentation
Date TBA
Board: PS05-09AM-421
Event Information
Poster Board
PS05-09AM-421
Poster
View posterAbstract
Developmental and epileptic encephalopathies (DEEs) are a subset of epileptic disorders defined as early onset, commonly severe and pharmaco-resistant epilepsies accompanied by several neurodevelopmental diseases such as cognitive decline or autism. A recent study analyzing the genome of several patients affected with DEEs linked AP2M1 to the emergence of the syndrome. AP2M1 encodes the µ-subunit of the AP-2 adaptor complex, which is involved in clathrin-mediated endocytosis, a mechanism crucial to synaptic vesicle component recycling. Since the expression of a dysfunctional variant of AP2M1 has already been tested in primary cell cultures, testing the modulation of AP2M1 at the histological level is of particular interest. Organotypic brain slice cultures (OBSCs) have the advantage of maintaining in vivo cellular architecture and cell type distribution. Here we show how AP2M1 modulation might have an effect on the electrophysiological activity of mouse OBSCs both at the single cell and at the network level using patch clamp and multi electrode array (MEA) recordings.
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