Poster preview
ePoster
MOTOR SYSTEM OLIGODENDROGLIA ATLAS REVEALS ACTIVATION STATES ASSOCIATED WITH REGION-SPECIFIC VULNERABILITY IN ALS
Maria Georgopoulouand 10 co-authors
CNL, VIB, Ku Leuven
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS05-09AM-268
Presentation
Date TBA
Board: PS05-09AM-268
Event Information
Poster Board
PS05-09AM-268
Poster
View posterAbstract
Oligodendrocytes, the second most abundant cell type in the motor cortex and the most abundant in the spinal cord—are increasingly recognized as active contributors to neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS). Yet their diversity and disease relevance in the central nervous system remain incompletely defined. Here, we generate a comprehensive single-nucleus transcriptomic atlas of 176 samples, integrating 2 in-house and 16 publicly available datasets. It encompasses more than 1,000,000 cells (350,000 oligodendrocytes and oligodendrocyte precursors) from the human motor cortex and spinal cord in non-neurological controls and ALS patients. We identify a previously unrecognized oligodendrocyte subpopulation—marked by PPEF1—and uncover regional differences, with distinct OPALIN+ and RBFOX1+ oligodendrocyte proportions between cortex and spinal cord. Importantly, we demonstrate that “disease-associated oligodendrocytes” (DAOs) represent a distinct activation state, rather than a distinct subpopulation. In ALS, spinal cord oligodendrocytes selectively shift toward reactive states, characterized by JUND-enriched regulon activity, heightened metabolic demand, increased intercellular signaling, and activation of cell-death pathways. Finally, we identify LRP2 as a novel transcriptional oligodendrocyte marker and find LRP2 and MBP proteins to be elevated in ALS cerebrospinal fluid (CSF), offering a potential biomarker of oligodendrocyte dysfunction. Our study provides a systems-level framework to interpret oligodendrocyte and OPC changes in ALS, linking cell state shifts to molecular alterations that can guide biomarker and therapeutic strategies.
Recommended posters
SPATIAL TRANSCRIPTOMICS REVEALS DISTINCT MOLECULAR SIGNATURE OF MOTOR NEURONS AND INTERNEURONS IN CONTROL AND PRE-SYMPTOMATIC STAGES OF AN ALS MOUSE MODEL
Manuela Rizzi, Roser Montañana-Rosell, Santiago Mora Parada, Sara Newell, Morten Nielsen, Kasper Thorsen, Ilary Allodi
CORTICAL NEURONS ARE NEURODEVELOPMENTALLY PRIMED TO DYSFUNCTION AND DEGENERATION IN AMYOTROPHIC LATERAL SCLEROSIS MOUSE MODELS
Marina Hernan Godoy, Aurélien Diebold, Geoffrey Stuart-Lopez, Charlotte Gorin, Verónique Devignot, Sylvie Grosch, Stéphane Dieterle, Pascal Kessler, Nathalie Brouard, Stéphanie Le Gras, Virginie Andry, Yannick Goumon, Caroline Rouaux
SPATIAL MULTIOMICS MAPPING OF NEUROINFLAMMATION IN A MOUSE MODEL OF AMYOTROPHIC LATERAL SCLEROSIS
Cansaran Saygili Demir, Josue Ballesteros Alvarez, Marion Bonnet, Kim Van Den Handel, Samuel Aubert, Pedro Pedro, Anna Laura Calvanese, Debia Wakhloo, Anushka Dikshit, Maria Giuseppina Procopio, Saska Brajkovic
STUDY OF OLIGODENDROCYTES DEGENERATION AND STUDY OF TRANSSPINAL REPETITIVE MAGNETIC STIMULATION IN SOD1 MOUSE MODEL OF ALS
Clémence Raimond, Laurine Moncomble, Fannie Semprez, Paganini Francesca, Pauline Neveu, Guérout Nicolas
MOLECULAR PROFILING OF MICROGLIA-ASTROCYTE CROSSTALK IN C9ORF72 ALS/FTD REVEALS DYSREGULATION OF CRITICAL SIGNALING PATHWAYS
Briana Ondatje, Lauren Gittings, Kim Preller, Lynette Bustos, Jennifer Levy, Eric Alsop, Ritin Sharma, Melissa Martinez, Krystine Mansfield, Nate Hansen, Bessie Meechoovet, Robert Culibrk, Ignazio Piras, Matthew Huntelman, Patrick Pirrotte, Kendall Van Keuren- Jensen, Rita Sattler
TOWARD PRECISION MEDICINE IN AMYOTROPHIC LATERAL SCLEROSIS: BLOOD-BASED TRANSCRIPTOMIC BIOMARKER DISCOVERY
Asif Anamta, Gulzar Fatima, Sekaran Karthik, Hatem Zayed