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MULTIPLE LIGANDS SYNERGISTICALLY ACTIVATE TRPV4 IN ASTROCYTES
Koji Shibasakiand 1 co-author
University of Nagasaki
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS03-08AM-501
Presentation
Date TBA
Board: PS03-08AM-501
Event Information
Poster Board
PS03-08AM-501
Poster
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We previously reported that TRPV4 is expressed in ~30% subpopulation of astrocytes in brain, and the TRPV4 activation leads to gliotransmitter (ATP and glutamate) release and increases synaptic transmission. It has been reported that TRPV4 is activated by various stimuli such as warmth temperature (>34°C), hypotonic stimulus, extracellular arachidonic acid and mechanical stimulus. All TRP channels have unique properties called synergistic effects. If we apply two different agonists, thresholds of each agonist can be effectively reduced. Thus, we can observe significant TRP channel activation by combination of two different agonists. These backgrounds indicate that TRPV4 can be potentiated by combinational application of two different agonists. In this study, we examined the possibility by an electrophysiological and a Ca2+-imaging experiment. Combinational application of several ligands significantly potentiated the TRPV4 activation. These results indicate that endogenous TRPV4 is strongly activated by multiple ligands, and lead to enhanced gliotransmitter release in naïve astrocytes.
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