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ePoster
MULTISCALE MAPPING OF AD PATHOLOGY IN THE HUMAN BRAIN
Lisa Broisinand 1 co-author
UNIGE
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS02-07PM-374
Presentation
Date TBA
Board: PS02-07PM-374
Event Information
Poster Board
PS02-07PM-374
Poster
View posterAbstract
Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by the stereotyped spread of pathological proteins through the brain, following specific neuronal networks. Although two-dimensional (2D) histological staging remains the diagnostic gold standard, the three-dimensional (3D) organization, dynamic evolution across disease stages and precise subcellular localization of these protein aggregates remains poorly understood.
Recent pre-clinical studies suggest a trans-synaptic propagation mechanism of pathological proteins. However, direct investigation in humans has been hindered by limited access to high-quality samples, imaging constraints, and tissue preservation challenges.
Here, we combine tissue clearing and expansion microscopy to map AD pathology across scales in human brain tissue, from entire brain regions to the nanoscale organization of synapses. This integrated approach offers access to region- and stage-specific ultrastructural changes in pathological proteins, and to their trans-synaptic spread within mesoscale-identified disease-affected brain regions.
By advancing the histological characterization of AD pathology in human tissues, this method supports human-based research and opens new perspectives for translational research in AD.
Recent pre-clinical studies suggest a trans-synaptic propagation mechanism of pathological proteins. However, direct investigation in humans has been hindered by limited access to high-quality samples, imaging constraints, and tissue preservation challenges.
Here, we combine tissue clearing and expansion microscopy to map AD pathology across scales in human brain tissue, from entire brain regions to the nanoscale organization of synapses. This integrated approach offers access to region- and stage-specific ultrastructural changes in pathological proteins, and to their trans-synaptic spread within mesoscale-identified disease-affected brain regions.
By advancing the histological characterization of AD pathology in human tissues, this method supports human-based research and opens new perspectives for translational research in AD.
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