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OMEGA-3 SUPPLEMENTATION MODULATES STRESS-INDUCED INCREASE IL-6 AND TLR4 GENE EXPRESSION
Sara Ventoand 4 co-authors
University of Valencia
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS02-07PM-457
Presentation
Date TBA
Board: PS02-07PM-457
Event Information
Poster Board
PS02-07PM-457
Poster
View posterAbstract
Social stress enhances proinflammatory signaling in the central nervous system via Toll-like receptor (TLR) activation, increasing interleukin-6 (IL-6) and promoting neuroinflammation. Omega-3 long-chain polyunsaturated fatty acids have anti-inflammatory effects, suggesting a role in modulating stress-induced neuroinflammation. This study examined whether an omega-3–enriched diet modulates Il-6 and Tlr4 gene expression following social stress.
Forty-eight OF1 mice (24 males, 24 females) were assigned to standard or omega-3 diets and to stress (social or vicarious social defeat) or exploration conditions. Diets began at postnatal day (PND) 21, stress exposure occurred on PND 47, 50, 53, and 56, followed by five weeks of voluntary ethanol consumption. Striatal and hippocampal tissues were collected on PND 122 for RT-PCR analysis.
Overall, males showed higher levels of IL6 in the striatum and TLR4 in striatum and hippocampus compared with females. In males, stress increased Il-6 gen expression in the striatum and TLR4 in the hippocampus, was reversed by omega-3 supplementation. However, the increased in Il-6 gen expression in the hippocampus, was not affected by omega-3 diet.
In females, stress-induced increase in Tlr-4 gen expression in the striatum and IL6 in the hippocampus was equally counteracted by omega-3. However, omega 3 did not affected stress-induced increased in Il-6 in the hippocampus.
Irrelevant of the stress exposure, omega 3 enriched diet decreased IL6 gen expression in striatum of male and female mice.
To sum up, stress increased IL6 and TLR4 gene expression, while omega-3 exerted a generally suppressive modulatory effect, supporting its use to mitigate stress-induced neuroinflammation.
Forty-eight OF1 mice (24 males, 24 females) were assigned to standard or omega-3 diets and to stress (social or vicarious social defeat) or exploration conditions. Diets began at postnatal day (PND) 21, stress exposure occurred on PND 47, 50, 53, and 56, followed by five weeks of voluntary ethanol consumption. Striatal and hippocampal tissues were collected on PND 122 for RT-PCR analysis.
Overall, males showed higher levels of IL6 in the striatum and TLR4 in striatum and hippocampus compared with females. In males, stress increased Il-6 gen expression in the striatum and TLR4 in the hippocampus, was reversed by omega-3 supplementation. However, the increased in Il-6 gen expression in the hippocampus, was not affected by omega-3 diet.
In females, stress-induced increase in Tlr-4 gen expression in the striatum and IL6 in the hippocampus was equally counteracted by omega-3. However, omega 3 did not affected stress-induced increased in Il-6 in the hippocampus.
Irrelevant of the stress exposure, omega 3 enriched diet decreased IL6 gen expression in striatum of male and female mice.
To sum up, stress increased IL6 and TLR4 gene expression, while omega-3 exerted a generally suppressive modulatory effect, supporting its use to mitigate stress-induced neuroinflammation.
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