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ePoster
RECOVERY OF MOUSE HIPPOCAMPAL NEUROPHYSIOLGY AFTER CRYOPRESERVATION BY VITRIFICATION
Fang Zhengand 2 co-authors
Friedrich-Alexander-Universität Erlangen-Nürnberg
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS05-09AM-535
Presentation
Date TBA
Board: PS05-09AM-535
Event Information
Poster Board
PS05-09AM-535
Poster
View posterAbstract
Cryopreserving brain tissue with traditional freezing methods is challenging due to ice crystal damage. Vitrification enables ice-free cryopreservation by solidifying tissue into an amorphous glass, usually via rapid cooling and cryoprotective agent (CPA) loading, yet its impact on neuronal viability and synaptic transmission remains to be characterized. Here, we performed electrophysiological recordings in adult mouse hippocampus slices, after vitrification of brain slices in vitro and of the whole brain in situ with optimized vitrification protocols. Whole-cell patch-clamp recordings from dentate gyrus granule cells and CA1 pyramidal cells post-vitrification revealed largely preserved neuronal excitability, manifested in typical cell membrane properties and action potential discharge. Spontaneous synaptic events, detected at resting membrane potential, did not differ between control and post-vitrification cells. Recovery of synaptic function in the hippocampus post-vitrification was further verified with extracellular field potential recordings of population synaptic responses to electrical stimulation. In addition to restored basic synaptic transmission, we found hippocampal long-term synaptic potentiation well-preserved, indicating that the cellular machinery of learning and memory remains operational. Thus, our findings extend the knowledge of biophysical limits for cerebral hypothermic shutdown, and demonstrate the recovery of brain functionality after complete cessation of molecular mobility in the vitreous state.
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