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SINGLE-CELL CHARACTERIZATION OF NOVEL GABAERGIC SUBTYPES IN THE DEVELOPING BRAINSTEM
Silvana Bodington Celmaand 3 co-authors
Molecular and Integrative Biosciences Research Programme (MIBS)
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS03-08AM-450
Presentation
Date TBA
Board: PS03-08AM-450
Event Information
Poster Board
PS03-08AM-450
Poster
View posterAbstract
The generation of diverse inhibitory neuron subtypes is critical for the formation of tegmental nuclei that regulate mood, motivation and movement. Using single-cell RNA sequencing, we identified distinct GABAergic neuron subtypes in the developing brainstem, including previously uncharacterized neuropeptidergic populations. We validated these populations and determined their anatomical distribution using multiplex RNAscope in situ hybridization. Our results refine the molecular and spatial landscape of GABAergic neuron diversity in the anterior brainstem and provide new insight into the developmental origins of inhibitory neuronal heterogeneity relevant to brainstem circuit function and behavior.
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