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SINGLE CELL TRANSCRIPTOMIC ANALYSIS ON OLIGODENDROCYTE LINEAGE CELLS SHOWS NEUROINFLAMMATION INDUCED IN MICE PUPS TARGET OLIGODENDROCYTES PRECURSOR CELLS (OPCS)
Lea Livramentoand 6 co-authors
University Paris Cite
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS07-10AM-140
Presentation
Date TBA
Board: PS07-10AM-140
Event Information
Poster Board
PS07-10AM-140
Poster
View posterAbstract
Neuroinflammation in premature infants is often accompanied by diffuse white matter injury (DWMI) caused by the blockade of oligodendrocyte precursor cells (OPC) differentiation and maturation leading to hypomyelination, which increased the risk for neurological disorders. We aimed at identifying the OPC cell populations that are the most affected by neuroinflammation. For this, we used an established mouse model of exposure to neuroinflammation (interleukin 1ß (IL1 ß) injections on mice neonates (Favrais et al., 2011, Schang et al, 2022)), we MACS-isolated oligodendrocytes lineage cells from mouse pup brains, and performed a single-cell multiomics analysis, combining transcriptomic and chromatin accessibility information from the same cell to interrogate cell identity (ID). First, we thus could refine our knowledge of the cell ID from each oligodendrocyte lineage subtype along the oligodendrocyte maturation trajectory. Second, by characterising their inflammatory response, we showed that the most immature cell subtypes are the most sensitive to stress and that inflammatory response pathways are predominantly up regulated in these vulnerable cell populations. We are currently identifying the transcription factor pathways at play using chromatin accessibility and explore the spatial organisation of these cell subtypes in vivo in mouse cortices in both sexes.
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