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TWO-PHOTON IMAGING OF ELECTRICAL MICROSTIMULATION-INDUCED NEURONAL ACTIVITY PATTERNS IN MOUSE V1
Matthieu Provansaland 6 co-authors
Netherlands Institute for Neuroscience
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS01-07AM-406
Presentation
Date TBA
Board: PS01-07AM-406
Event Information
Poster Board
PS01-07AM-406
Poster
View posterAbstract
Brain machine interfaces represent a promising technology to restore sight in blind individuals. These devices rely on intracortical electrodes which activate the surrounding neurons, leading to the perception of artificial dots of light. Many parameters can shape the electrical stimulation and therefore impact the neuronal activation spread and corresponding visual percept. We therefore need to understand how these parameters control the activated neuronal population.
To this end, we have developed a pipeline combining electrical microstimulation and 2-photon imaging in mice to compare the excitatory neurons activation patterns elicited by different stimulation parameters.
Among those parameters, double electrode bipolar stimulation steers the current between electrodes compared to double electrode monopolar stimulation. Our results show this current steering does not segregate the activity between the electrodes. Instead, the main changes triggered by bipolar stimulation resides in the decrease of neuronal activation associated with the switch of one cathode-leading electrode to an anode-leading electrode.
This work has important implications for the development of next-generation visual prosthetics.
To this end, we have developed a pipeline combining electrical microstimulation and 2-photon imaging in mice to compare the excitatory neurons activation patterns elicited by different stimulation parameters.
Among those parameters, double electrode bipolar stimulation steers the current between electrodes compared to double electrode monopolar stimulation. Our results show this current steering does not segregate the activity between the electrodes. Instead, the main changes triggered by bipolar stimulation resides in the decrease of neuronal activation associated with the switch of one cathode-leading electrode to an anode-leading electrode.
This work has important implications for the development of next-generation visual prosthetics.
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