Cryo-FIB Research Specialist

Run cryo-FIB/SEM, cryo light microscopy and CLEM, and advanced cryo-EM sample preparation in Janelia's CryoEM Shared Resource, which serves the whole HHMI community with four Titan Krios microscopes, two cryo-FIB/SEM systems (Aquilos 2 and Helios Hydra) and cryo light microscopes. Most of the role is routine operation: preparing samples, FIB milling, imaging and collecting data for facility users and collaborators, keeping instrument records, judging sample and image quality, and troubleshooting hardware, software and technique.

It also includes consulting with researchers on experimental design, building or adapting protocols from sample preparation through milling, imaging and data correlation, bringing new methods into the facility and training users and staff. Hiring range: Research Specialist I USD 71,760-89,700; Research Specialist II USD 83,760-104,700 per year.

Hands-on experience preparing samples, milling or imaging on a cryo-FIB/SEM or cryo-EM system, and the ability to operate and troubleshoot instruments such as a Titan Krios or Helios Hydra. Meticulous record-keeping, sound judgement about sample and image quality, ability to teach complex procedures, and comfort building new protocols. Image-correlation or 3D visualisation software and experience training researchers are a plus.