From Metabotypes to Mechanisms: Integrative Omics of Gut Microbiota–Bile Acid–Host Crosstalk in Cholangiocarcinoma

Investigate interactions between the gut microbiome, bile acids and cholangiocarcinoma through metabolic phenotyping, sequencing and multivariate analysis. Human faecal metabolomics identifies disease-associated patterns, followed by a microbiota-transplantation model combining NMR and long-read 16S measurements. The work examines how disease-associated microbiota and bile reinfusion reshape microbial and systemic metabolism and generate testable biomarker and intervention hypotheses.