Dorsal Raphe
dorsal raphe
Organization of Midbrain Serotonin System
The serotonin system is the most frequently targeted neural system pharmacologically for treating psychiatric disorders, including depression and anxiety. Serotonin neurons of the dorsal and median raphe nuclei (DR, MR) collectively innervate the entire forebrain and midbrain, modulating diverse physiology and behaviour. By using viral-genetic methods, we found that DR serotonin system contains parallel sub-systems that differ in input and output connectivity, physiological response properties, and behavioural functions. To gain a fundamental understanding of the molecular heterogeneity of DR and MR, we used single-cell RNA - sequencing (scRNA-seq) to generate a comprehensive dataset comprising eleven transcriptomically distinct serotonin neuron clusters. We generated novel intersectional viral-genetic tools to access specific subpopulations. Whole-brain axonal projection mapping revealed that the molecular features of these distinct serotonin groups reflect their anatomical organization and provide tools for future exploration of the full projection map of molecularly defined serotonin groups. The molecular architecture of serotonin system lays the foundation for integrating anatomical, neurochemical, physiological, and behavioural functions.
Serotonergic activity in the dorsal raphe nucleus through the lens of unsupervised learning
COSYNE 2025
Serotonergic neurons in the dorsal raphe regulate visual attention
COSYNE 2025
Acute and chronic treatment with the nitric oxide synthase inhibitor agmatine stimulates serotonergic neurons in the rat dorsal raphe nucleus
FENS Forum 2024
An alternative to treat depression-like behaviors: The effects of S-mecamylamine in the dorsal raphe nucleus
FENS Forum 2024
Depressive and anxious phenotype correlates with functional changes in the ventromedial prefrontal cortex - dorsal raphe nucleus circuit in female mice with alpha-synucleinopathy
FENS Forum 2024
Dorsal raphe nuclei/ventrolateral periaqueductal grey and cerebellar fastigial nucleus interactions modulate danger response during fear learning
FENS Forum 2024
Involvement of dorsal raphe nucleus (DRN) astrocytes in valence processing
FENS Forum 2024
Mapping the projections of serotonergic dorsal raphe neurons in zebrafish
FENS Forum 2024
Neuronal signature of cognitive flexibility in the prefrontal-dorsal raphe circuit
FENS Forum 2024
Noxious stimulus-responsive neurons in the ventral periaqueductal gray and dorsal raphe nucleus
FENS Forum 2024
Role of the serotonergic dorsal raphe nucleus in mediating the effects of developmental chronic stress in zebrafish
FENS Forum 2024
Serotonin neurons in the dorsal raphe nucleus encode probability rather than value of future rewards
FENS Forum 2024
Topographically organized sensory-motor activity of dorsal raphe and its forebrain innervations modulate resting-state and sensory-evoked forebrain activity and animal behavior
FENS Forum 2024