Topic: acousto-optic deflectors

To study the neural correlates of naturalistic behaviors, we seek to record neural activity in unrestrained animals. Due to behavior-induced motion artifacts, such recordings are particularly challenging for two-photon fluorescence microscopy. Studying multi-neuronal activity in the central nervous system in moving larval Drosophila, whose brain is translated, rotated, and deformed in 3D, has been a great challenge. To overcome the challenges, we developed a method capable of calcium recording from multiple neurons in a brain of a freely moving Drosophila larva with extremely low latency (100

Although dendritic signaling strongly influences sensory processing and learning, the connection between dendritic integration and somatic output is still not fully understood. Our aim is maping the origins of dendritic events during different aspects of visual learning.We used 3D two-photon laser scanning microscopy with acousto-optic deflectors to simultaneously record jRGECO1a (calcium) and JEDI-2p (voltage) signals in the apical dendrites of layer 5 pyramidal cells in the primary visual cortex of mice during visual discrimination. We examined mice with controlled water access participating

The state-of-the-art techniques for all-optical interrogation of neuronal circuits in vivo typically combine two-photon (2P) calcium imaging and digital holography to precisely activate target neurons with sculpted light patterns. This approach is however limited in speed and in throughput, ultimately due to the intrinsic constraints of digital holography. We employed acousto-optic deflectors (AOD)-based 2P approaches (“ULoVE”, Villette et al. 2019; “3D-CASH”, Akemann et al. 2022) to simultaneously record and perturb neuronal activity in awake head-fixed mice across single planes or three-dime