Topic: β-arrestin

DREADDs have revolutionized neuroscience research and hold promise for therapeutic applications. While their ability to modulate neuronal activity is well established, the specific signaling pathways they can trigger remain only partially explored. In this project, we therefore examine the signaling preferences of the human muscarinic acetylcholine receptor M3 and M4 (hM3/4) and the respective DREADDs (hM3D and hM4D). Additionally, we evaluate the impact of the individual single point mutations Y3.33C and A5.46G, which convert the wild type receptors to the DREADD. We investigated signaling of

Complex interactions between serotonin (5-hydroxytriptamine) and orexin (hypocretin) systems in the CNS modulate diverse physiological processes, including mood regulation, energy homeostasis, and spontaneous physical activity. A portion of this interaction may be explained by G protein-coupled receptor oligomerization, which might influence agonist affinity, efficacy, trafficking, and signal transduction. Orexin receptor 1 (OX1) and 5-hydroxytriptamine 2C receptor (5-HT2C) co-express in diverse neuronal populations and are involved in pathological conditions such as anxiety and eating disorde