Topic: Brain Imaging Data Structure

Light-Sheet Fluorescence Microscopy (LSFM) is an excellent tool to study neuronal populations in large samples such as the whole mouse brain, thanks to its inherent three-dimensional nature and its high resolution down to the single cell. In this work we report on our latest technological developments in both hardware and software and their applications in several projects aiming at reconstructing the neuronal organization in both the human cortex and in whole mouse brain.Our latest LSFM setup is capable of simultaneous excitation and acquisition at four wavelengths, thus enabling fast reconst

A key goal underlying the FAIR principles is to enhance reusability of research data. Yet in the field of animal neurophysiology this is challenging due to a high degree of heterogeneity and complexity in the experimental instruments, techniques and analytic tools used. Consequently, this diversity leads to different approaches to data organisation, a wide range of file formats and countless variations of lab-specific metadata that can be difficult to interpret, ultimately preventing efficient data reuse. Here we propose a workflow for standardising and sharing datasets from different electrop