Topic: brain microvascular endothelial cells

Organ-on-chip (OoC) technologies are designed to mimic natural structure and function of organs. Integrating microfluidics to OoCs allows long-term perfusion and stability, essential for cultivating physiological conditions similar to intact organs. However, current OoC systems predominantly use primary cell cultures over organotypic tissue cultures (OTCs) or entire organs, due to the absence of extensive microvasculature. This limitation compromises the viability of larger tissue volumes as they suffer from inadequate oxygen and nutrient delivery, along with the absence of perfusion. This stu

The cerebral capillary bed consists of a dense network of vessels out of specialized brain microvascular endothelial cells (BMECs) that deliver essential nutrients to the brain. To form functionally branched vasculature, BMECs have to sense and adapt to site-specific shear stress, relayed by mechanosensory cell surface proteins (CSPs) presented on the BMEC surface. Previous research on the effect of physiological and pathological shear stress on large vessels has studied unidirectional and oscillatory flow as proxies for laminar and disturbed shear stress. However, the effect of shear stress o