Topic: calcium recording

To study the neural correlates of naturalistic behaviors, we seek to record neural activity in unrestrained animals. Due to behavior-induced motion artifacts, such recordings are particularly challenging for two-photon fluorescence microscopy. Studying multi-neuronal activity in the central nervous system in moving larval Drosophila, whose brain is translated, rotated, and deformed in 3D, has been a great challenge. To overcome the challenges, we developed a method capable of calcium recording from multiple neurons in a brain of a freely moving Drosophila larva with extremely low latency (100

Memories have been suggested to be stored in groups of neurons known as neuronal ensembles, or “engrams”, and neuroplasticity is believed to be the underlying mechanism that allows the formation or remodeling of these ensembles, thus allowing memory modifications. Critically, the hippocampus has been described as a central hub for neuronal ensembles. In the context of fear memory, the representation of the hippocampal neuronal ensemble has been characterized, while little is known about the neuronal ensemble within the hippocampus and the upstream circuits in spatial learning and memory. With