Topic: cell surface proteomics

Animals exhibit a stunning array of natural behaviors, many of which are innate and controlled by intricate neural circuits genetically specified. In the fruit fly, connectomics work has revealed precise patterns of neuronal connectivity and their computational potential. However, we lack a molecular understanding of how such circuit connectivity is established with synaptic specificity, in part because it remains challenging to define the molecular landscape and interactions at subcellular resolution in intact tissue. Taking advantage of the well-defined and accessible Drosophila motion visio

During development, the specificity of retinal neural circuit wiring is established by developmental programs that require cell adhesion molecules (CAMs) to define precise layering and connectivity. However, our current understanding of this process remains superficial, as the field has been guided primarily by gross anatomical phenotypes that can be visually quantified. Here, we present an alternative approach to studying circuit wiring that focuses on functional changes rather than relying on morphological ones. To this end, we use the mouse retina as a model and employ a recently developed