Topic: cell viability

Glioblastoma multiforme (GBM) is a highly malignant brain tumor with poor prognosis after conventional treatment. Therefore, potential treatment strategies and novel therapeutic targets have gained increased attention. Vanilla planifolia is a source of vanilla flavoring due to its high vanillin content, and several studies have shown its antitumor activity. Our data revealed that an ethanol extract of vanilla planifolia stem (VAS) at 200 ng/μl significantly reduced cell viability and colony formation in GBM cells. VAS also induced autophagy, as shown by MAP1LC3 cleavage. Further RNA-seq analys

Neuronal networks derived from human induced pluripotent stem cells (hiPSCs) have gained attention for their potential use in in vitro modeling of epilepsy and seizure-like electrophysiological activity. Similar to in vivo animal models, seizure-like activity can be induced in these neuronal cultures using kainic acid (KA), direct agonist of the glutamate receptors. However, to establish more relevant in vitro model using KA, better understanding of KA mediated acute and prolonged effects on human neuronal networks is required. In this study, we investigated both acute and long-term KA concent