Topic: cerebral small vessel disease

The effect of post mortem delay, age of the subjects and fixation protocols were examined on immunostaining of the human brain samples.Short post-mortem time (2-5h) is crucial for the quality of human brain samples. Our group uses cerebral cortical samples and hippocampi with this short post-mortem period, which are suitable for fine structural research. Here the advantages and disadvantages of perfusion and immersion fixation were investigated, and the quality of different immunohistochemical stainings (calbindin, calretinin, parvalbumin, nonphosphorylated neurofilament H /SMI32/ among others

Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a hereditary cerebral small vessel disease caused by NOTCH3 gene mutations affecting cysteine residues in the Notch3 receptor's extracellular domain. The disease is characterized by recurrent strokes, cognitive decline, migraines with aura, epilepsy, gait disturbances, and psychiatric problems. This study investigated the clinical features and NOTCH3 mutations in Cypriot CADASIL patients to identify genotype-phenotype correlations. Nineteen genetically confirmed CADASIL patients (10 males,

Methylglyoxal (MGO), a by-product of glycolysis and major precursor in the formation of advanced glycation endproducts (AGEs), is increased during ageing and in diabetes and plays a role in peripheral microvascular dysfunction. Whether MGO affects the brain microvasculature is unknown. The aim of this study is to investigate whether higher plasma MGO concentrations are cross-sectionally associated with markers for cerebral small vessel disease (CSVD) and with cognitive impairment. In participants of the Maastricht Study, a population-based cohort study oversampled with type 2 diabetes (age=59.

AIM: Hypertension is known to impair microcirculation in the brain, leading to cerebral small vessel disease (CSVD), which correlates strongly with cognitive impairment. Studies have shown a high prevalence of hypertension-related cranial MRI lesions, including arteriosclerosis and various forms of brain damage. Pericytes, a component of the neurovascular unit, are implicated in controlling blood flow and have been linked to Alzheimer's disease pathology, but their role in hypertension-related microcirculation disorders and vascular dementia has not been extensively studied. The study aims to